Journal article
Long-Read RNA Sequencing Identifies Polyadenylation Elongation and Differential Transcript Usage of Host Transcripts During SARS-CoV-2 In Vitro Infection
JJY Chang, J Gleeson, D Rawlinson, R De Paoli-Iseppi, C Zhou, FL Mordant, SL Londrigan, MB Clark, K Subbarao, TP Stinear, LJM Coin, ME Pitt
Frontiers in Immunology | Published : 2022
Open access
Abstract
Better methods to interrogate host-pathogen interactions during Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections are imperative to help understand and prevent this disease. Here we implemented RNA-sequencing (RNA-seq) using Oxford Nanopore Technologies (ONT) long-reads to measure differential host gene expression, transcript polyadenylation and isoform usage within various epithelial cell lines permissive and non-permissive for SARS-CoV-2 infection. SARS-CoV-2-infected and mock-infected Vero (African green monkey kidney epithelial cells), Calu-3 (human lung adenocarcinoma epithelial cells), Caco-2 (human colorectal adenocarcinoma epithelial cells) and A549 (human lung ..
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Awarded by Stimson Miller Foundation
Funding Acknowledgements
This research was supported by a University of Melbourne "Driving research momentum" award (to LC) and NHMRC EU project grant (GNT1195743 to LC). KS was supported by an NHMRC Investigator grant. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza was supported by the Australian Government Department of Health. MC was supported by an Australian National Health and Medical Research Counci l Investigator Fellowship (APP1196841). JC was supported by the Miller Foundation and the Australian Government Research Training Programme (RTP) scholarship.